Citruslabs TruDiagnostic™ Blood-verified clinical trial · Aug 2025

Our Clinical Studies

Longevity Shots.
The study results.

Two independent Citruslabs studies. One measured biological age, skin and energy over 3 months. The other tracked how participants felt after 5 weeks.

Conducted by

Citruslabs Research Team

Biological age testing

TruDiagnostic™ TruAge

Statistical method

Independent · p<0.05

47%

3-month clinical trial

Nearly 1 in 2 daily users measured biologically younger.

After 3 months, 47% of daily users had a lower biological age than at the start, measured through TruDiagnostic™ blood testing.

Source · Citruslabs / TruDiagnostic™ Aug 2025

3-month study · Daily users

The results at a glance.

Blood test

47%

had a younger biological age.

TruDiagnostic™ · 3-month follow-up

Every marker we tracked

19/19

signs of aging moved in the right direction.

All changes outside the margin of chance

Energy

+78%

improvement in energy satisfaction scores.

3 months vs baseline

Body aging

+52%

improvement in body-aging satisfaction scores.

3 months vs baseline

Verified by Citruslabs
TruDiagnostic™ Epigenetic Lab
Harvard-trained MD
View Citruslabs case study

Clinical trials

Two independent studies. Same product.

Different studies, different measurements. Blood testing measured biological age. Participant surveys measured how people felt.

Study 1 · 3 months

Biological age, skin and energy

Format
Two groups: daily users and twice-weekly users.
Duration
3 months · check-ins at month 1, 2, and 3.
Participants
Women who came in with fatigue, dull skin, or low drive.
What we measured
Biological age via blood test (TruDiagnostic™). Skin changes by board-certified dermatologist. 19 signs of aging rated by participants.
Analysis
Independent statistical analysis confirmed significance.
By
Citruslabs · Las Vegas, NV · 2025.

Top finding

Every marker we tracked improved. 47% came out biologically younger by month 3.

Study 2 · 5 weeks

How participants felt

Format
Daily use for 5 weeks.
Duration
5 weeks · check-ins each week.
Participants
Women who came in with concerns about their skin, energy, or vitality.
What we measured
How women felt about their skin, energy, fatigue, mental sharpness. week by week.
Method
Weekly self-reported ratings from baseline to week 5.
By
Citruslabs · April 2025.

Top finding

84% wanted to keep taking it. Skin satisfaction went from 12% to 79%.

Methodology

How the 3-month trial worked.

01

Baseline taken.

Blood test, dermatologist photos, and a survey on 19 signs of aging. The starting point for every participant.

Day 0

02

Daily use, 3 months.

No other changes to their routine, supplements, or treatments.

3 months

03

Tracked monthly.

Same survey at month 1, 2, and 3. Dermatologist re-reviewed photos and blood was re-tested at month 3.

M1 · M2 · M3

04

Independent analysis.

Citruslabs ran the numbers. The dermatologist scored photos without seeing self-reports. so the eyes and the data couldn’t lean on each other.

Confirmed significance

From our Chief Medical Officer

“When half of women drop their biological age in 3 months, you stop calling that a feeling. You call it measurement.”

The blood test isn’t a survey. It reads something your body is actually doing. When that number moves alongside every other marker we tracked, you stop wondering if it works. The mechanism is what we expected: replace the NAD+ your body stops producing, and your cells have what they need. for energy, for skin, for the brain.

Dr. Daniel Yadegar, MD Chief Medical Officer · Harvard-trained

Every parameter, side by side

Fourteen markers. Every one up.

Change in self-reported scores after 3 months of daily use, compared with baseline.

Track 01 · Skin

7 markers
Skin youthfulness satisfaction +48.9%↑
Skin appearance satisfaction +48.1%↑
Skin hydration +43.0%↑
Skin elasticity +35.4%↑
Skin firmness +29.4%↑
Overall skin complexion +24.4%↑
Fine lines & wrinkles +21.4%↑

A board-certified dermatologist independently confirmed visible improvement in skin dryness for nearly half of daily users by month 3.

Track 02 · Energy & mind

7 markers
Energy level satisfaction +78.2%↑
Maintain energy through day +57.3%↑
Body aging satisfaction +52.2%↑
Ability to focus +48.5%↑
Fatigue (less) +41.1%↑
Energy levels +38.9%↑
Alertness +31.0%↑

By month 3, almost 3 in 4 women said they had less fatigue, felt more alert, wanted to continue using it, and would recommend it to a friend.

View the complete results

The full table · Daily users · 3 months

The complete results

Self-reported ratings on a 1-to-5 scale, at baseline and month 3. Every change shown is statistically significant (p<0.05).

Track 01 · Skin

Hydration · firmness · youthfulness
ParameterBaselineMonth 3Change
Overall skin complexion2.963.68+24.4% ↑
Skin firmness2.883.73+29.4% ↑
Skin elasticity2.723.68+35.4% ↑
Skin hydration2.483.55+43.0% ↑
Fine lines & wrinkles2.923.55+21.4% ↑
Skin appearance satisfaction2.243.32+48.1% ↑
Skin youthfulness satisfaction2.323.45+48.9% ↑

A board-certified dermatologist independently confirmed visible improvement in skin dryness for nearly half of daily users by month 3.

Track 02 · Energy & mind

Fatigue · focus · vitality
ParameterBaselineMonth 3Change
Fatigue (1=worst → 5=best)2.323.27+41.1% ↑
Energy levels2.163.00+38.9% ↑
Maintain energy through the day2.083.27+57.3% ↑
Ability to focus2.483.68+48.5% ↑
Alertness2.883.77+31.0% ↑
Energy level satisfaction1.763.14+78.2% ↑
Satisfaction with body aging2.243.41+52.2% ↑

By month 3, almost 3 in 4 women said they had less fatigue, felt more alert, wanted to continue using it, and would recommend it to a friend.

Why we tested Longevity Shots

Why we tested it

Most supplements never get tested. We thought yours should be.

Longevity research was built on male physiology. Supplement marketing was built on rat models and confident voiceovers. We decided to find out if ours actually worked. in women, in real life, measured by an outside lab.

01 / Women, not lab averages

Tested on the body the product was made for.

Every woman in both studies came in with the same things: fatigue, dull skin, less drive. NAD+ is the cellular fuel they had less of. We didn’t test this on 25-year-old marathoners.

02 / Blood, photo, survey

Three kinds of evidence, in that order.

A blood test measured what the cells were doing. A board-certified dermatologist reviewed before-and-after photos. Women rated 19 signs of aging at every check-in. An outside statistician confirmed the changes weren’t chance.

03 / The full table, not the highlight reel

If you’re going to trust the science, you should see the data.

Every measurement, baseline, month-3 result and change is on this page. The Citruslabs study is also published independently at citruslabs.com. You don’t have to take our word for any of it.

Understand the key terms

Key terms

What the words mean.

Quick definitions for the science used in both studies.

NAD+
Nicotinamide adenine dinucleotide. A molecule every cell uses to make energy. NAD+ levels drop with age, which is one of the reasons fatigue and slower cellular repair show up over time.
Biological age
How old your body appears to be functioning, based on cellular markers. Different from chronological age (the number on your ID). Measured here through DNA methylation patterns in a blood test.
TruDiagnostic™
An FDA-registered laboratory in Lexington, Kentucky that performs biological-age testing from fingerprick blood samples. Used as the independent measurement lab in our 3-month clinical trial.
Citruslabs
An independent consumer-research lab in Santa Monica and Las Vegas that designs and runs clinical and perception studies for supplement and wellness brands. Citruslabs designed, ran, and analyzed both Happy Aging studies independently.
Liposomal NAD+
NAD+ encapsulated in tiny lipid spheres for absorption. The Longevity Shot uses a liposomal NAD+ booster with nicotinamide riboside chloride (a direct NAD+ precursor) plus resveratrol-enriched yeast.
Dual-arm trial
A study design that compares two groups taking the product on different schedules. The 3-month clinical trial split participants into a daily group and a twice-weekly group.
Likert scale
A 1-to-5 rating scale (strongly disagree → strongly agree) used in the surveys. Participants rated 19 signs of aging at every check-in.
Statistically significant
A change unlikely to be the result of chance. The clinical trial used a p<0.05 threshold. meaning each reported change has less than a 5% probability of being random.
Happy Aging NAD Longevity Shots

The product the data is about

NAD Longevity Shots.
5 weeks to feel it.

A liquid NAD+ shot with resveratrol and 12 supporting ingredients. One a day, with breakfast. The exact formula women drank in both studies above.

$54 $70
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Independent verification & partners

Citruslabs TruDiagnostic GraphPad Prism Harvard Medical